Inulinase Immobilized Lectin Affinity Magnetic Nanoparticles for Inulin Hydrolysis.

Abstract

In this presented paper, concanavalin A-modified cysteine-functionalized Fe3O4/Ag core/shell magnetic nanoparticles were synthesized and used as a support material for inulinase enzyme, which has been intensively used for the preparation of high-fructose syrup by hydrolyzing inulin. Inulinase adsorption capacity of Con A-functionalized Ag-coated magnetic nanoparticles was optimized by changing medium pH, temperature, and initial inulinase concentration, and maximum inulinase adsorption capacity was found to be 655.32 mg/g nanoparticle by using 1.00 mg/mL of inulinase solution in pH 3.0 buffer system at 25 °C. Finally, efficient inulin degradation capacity of the inulinase immobilized magnetic nanoparticles was demonstrated by TLC studies and released fructose amount was determined as 0.533 mg/mL only within the 5 min of hydrolysis. This newly developed hydrolysis strategy holds considerable promise to produce high-fructose syrup in many industries.