Abstract
Molecular epidemiology methods are useful to investigate cases of legionellosis. As Legionella pneumophila is widely distributed in the aquatic environment, its isolation from water samples does not allow, by itself, to identify the contamination source. Several molecular methods can be used : plasmid analysis, ribotyping, restriction enzyme analysis, pulsed field gel electrophoresis, arbitrarily primed-PCR, amplified fragment lengh polymorphism, infrequent restriction site PCR, sequencing. These methods should always be associated with an epidemiological investigation and should be validated in a given area on a large set of strains non epidemiologicaly linked.
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