Abstract
IT has recently been discovered that some eukaryotic genes are split, the mRNA coding sequences (exons) being interupted by intervening sequences (introns) of unknown function1–3. In all cases examined, these split genes are transcribed into an RNA precursor which is then matured by a splicing mechanism4–6. Because this type of gene structure has not previously been found in bacteria, it is generally believed that split genes cannot be expressed in Escherichia, but there are no facts to support this assumption. We demonstrate here that the presence of introns in the chicken ovalbumin gene prevents ovalbumin synthesis in E. coli K12.
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