Intron length polymorphism of actin genes in different varieties of ukrainian selection flax

Abstract

Aim. Intron length polymorphism (ILP) is an effective tool to identify genotypes and a reliable source of information with high interspecies and intraspecies variability. As the flax is one of the most widespread crops in Ukraine, to ex-plore the various members of the genus Linum L. are very useful. The main aim of this paper is to develop of molecular genetic markers based on the variability of flax actin genes to detect intron length polymorphism in different varieties of Ukrainian selection flax. Methods. CTAB-method for the isolation of DNA was used. Intron length polymorphism was identified by PCR amplification with specific primers, electrophoretic analysis under non-denaturing polyacrylamide gel was done. Results. 16 varieties of Ukrainian selection flax have been analyzed. It was shown that polymorphic am-plicons were available in analyzed plants. The lengths of fragments corresponding actin introns have varied from 676 bp to 1530 bp. Conclusions. The analysis of 16 varieties of Ukrainian selection flax demonstrated the ability of this method to detect the difference between genotypes at intraspecies level. From these results we can make a conclusion that method based on actin intron length polymorphism can be a source of useful information about genetic differentia-tion of taxonomic groups of plants.Keywords: molecular marker, actin, ILP (intron length polymorphism), Linum usitatissimum L.