Introgression of Phaseolus acutifolius A. Gray Genes into the Phaseolus vulgaris L. Genome

Abstract

AbstractThe tepary bean (Phaseolus acutifolius A. Gray) is a desirable genetic resource for incorporation of improved disease, pest, and stress resistance into common bean (P. vulgaris L.). Reproductive barriers separate the two species and the degree to which tepary genes may be introgressed into the common bean genome has not been well described. Greenhouse studies of gene introgression through recurrent back‐crossing to common bean were performed using two first backcross (BC1) and nine second backcross (BC2) populations. The truncate primary leaf characteristic of tepary bean was readily observed but the short primary leaf petiole and narrow bract tepary traits were infrequently observed in both BC1 and BC2 populations. In one BC, population high frequencies of adaxial stomata (characteristic of P. acutifolius) and the presence of a 30 kD cotyledon polypeptide from P. acutifolius were also observed; however, a diapho‐rase isozyme from P. acutifolius appeared to be eliminated from BC1 progeny at a high rate whereas 6‐phosphogluconate dehydrogenase alleles appeared to be transmitted normally. The expression of tepary primary leaf truncate morphology and bract width were correlated with decreased fertility in one of the two BC1 populations. Given suitable parental genotypes and population sizes it should be possible to transfer genes from P. acutifolius to P. vulgaris, especially in regions of the genome which are not associated with inviability or sterility. It will be difficult to transfer factors from certain regions of the tepary genome which are preferentially eliminated during introgression.