Introgressing Blast Resistant Gene <i>Pi-9(t)</i> into Elite Rice Restorer Luhui17 by Marker-Assisted Selection

Abstract

In this research, we introgressed the broad-spectrum blast resistant gene Pi-9(t) from the donor parent P2 into hybrid restorer Luhui17 by using backcross approach and molecular marker-assisted selection (MAS) technique. We detected the target gene through the specific molecular marker pB8 that close linked to the blast resistant gene Pi-9(t). As a result, sixty eight lines carrying broad-spectrum blast resistant gene Pi-9(t) were obtained, and then four lines, WR1023, WR1043, WR1056 and WR1062, were identified to be homozygous backcross lines with resistant gene Pi-9(t) by molecular phenotyping identification. Furthermore, the lines, WR1023 and WR1056 lines, were proved to have genetically stable agronomic characteristics, that demonstrated good plant architecture, high blast resistance and stable restoring ability, Therefore we suggested that these lines should be used as resistant gernplasms in in rice hybrid breeding program.