Introgession of powdery mildew resistance into cultural pea from wild accession of P. fulvum

Abstract

Wildlife pea specimen P. fulvum i-609881 of the UIP collection (Saint Petersburg, Russia) in a greenhouse box has demonstrated full resistance to powdery mildew. As a result of crossing with cultivated peas, interspecific hybrids of Stabil × i-6098881 were obtained. In populations of interspecific hybrids, the BC2F3 Stabil × i-609881 line of the leafless morphotype (af), resistant to the powdery mildew pathogen, was isolated as a result of backcrossing. As a result of crossing plants of this line with susceptible plants of the leafy (Af) variety Temp, the following series of pea hybrids was obtained. Analysis of F1 hybrids showed that all plants were characterized by resistance to the pathogen powdery mildew. In the population of F2 hybrids, a splitting analysis was performed on the basis of resistance to powdery mildew. The analysis revealed that the hybrid population consisted of 66 resistant and 20 affected plants. The actual splitting corresponded to the expected one for monogenic inheritance with a phenotype ratio of 3: 1 phenotype ratio (η05 2 test, p=0.7087438). Based on the analysis of populations of F1 and F2 hybrids, it is concluded that resistance to powdery mildew is the result of introgression of the genetic resistance factor from the genome of the P. fulvum i-609881 sample and is encoded by the dominant allele of a single gene.