Introductory remarks on glycoconjugate histochemistry

Abstract

In these opening remarks, many typical methods of demonstrating glycoconjugates were presented. Several such classical demonstration methods at the electron microscopic level have been revived to achieve a sufficient electron density, which makes it possible to use these methods routinely at that level. To detect sugar residues at the electron microscopic level, labeled lectins were used by means of preembedding and postembedding staining. Combining these methods, sugar residues in relation to cell organellae have been successfully detected. Many such examples were reported in this presentation.