Introduction of soybean into maize field reduces N2O emission intensity via optimizing nitrogen source utilization

Abstract

The application of synthetic nitrogen (N) fertilizer has increased anthropogenic N2O emission in global croplands. Due to the advantage of biological nitrogen fixation (BNF), the introduction of legume may lessen N2O emission intensity (emission/yield) in cereal field. Yet, this speculation lacks strong evidences and the relevant mechanistic explanations. A two-year field study was conducted using static chamber method (15N isotope labeling in fertilizer) in the mono- and inter-cropping fields with soybean introduced in maize field in semiarid rainfed Loess Plateau. The results indicated that maize-soybean intercropping showed 13 % lower N2O emission intensity than monocropping, attributed to 14 % and 20 % lower annually N2O emissions in intercropped zone than that of sole maize and soybean zones respectively. Also, a 15 % higher grain yield was observed in intercropped maize relative to that in sole maize. Particularly, during the peak time of emission following N fertilization and BNF within growing season, soybean introduction reduced 25 % and 24 % of N2O emissions in intercropped zone than sole maize and soybean zone respectively. It also favored maize plant with an additional 17 % more fertilizer-derived N, 21 % less soil-derived N and 30 kg N ha−1 soybean transferred-N from BNF. The contributions of N fertilization, BNF and soil initial organic N to N2O production were lessened in intercropping. In this case, the capacity of substrates N transformation into N2O emissions was lowered. Critically, soybean introduction reduced soil ammonia oxidation (as evidenced from amoA AOA and AOB gene abundance) in intercrops’ rhizosphere, and increased soil nitrite reduction (nirS and nirK gene abundance) in maize rhizosphere, and improved soil N2O reduction to N2 (nosZ gene abundance) in soybean rhizosphere. Therefore, soybean introduction optimized N source use for lower N2O emission intensity, via reducing the transformation of substrate N into N2O, and modifying the expression of nitrification and denitrification functional genes.