Introduction of an interface layer on hydroxyapatite whisker/poly(L-lactide) composite and its contribution for improved bioactivity and mechanical properties

Abstract

A hydroxyapatite whisker (w-HA) was synthesized via dissolution-precipitation by forming calcium-ethylene diamine tetra acetic acid (Ca-EDTA) complexing. The hydroxyapatite whisker was formed with precipitation of Ca2+ along the c-axis due to the space inhibition of Ca-EDTA complex during refluxing. The op-w-HA (oligomeric poly(lactic acid) modified w-HA), p-w-HA (poly(L-lactide) modified w-HA) and pc-w-HA (poly(L-lactide) and cyclodextrin modified w-HA) were obtained via the surface modification of w-HA. The particle size, surface charge and biocompatibility of theses modified w-HA particles were successfully adjusted. Among these materials, pc-w-HA exhibited nearly no toxicity, better adhesion to mesenchymal stem cells (MSCs) (5 times better than w-HA) and greater osteoinductivity among the obtained materials (40% of mineralized extracellular matrix higher than w-HA) due to better surface properties. Different kinds of powders (w-HA, p-w-HA and pc-w-HA) were blended with PLLA (poly(L-Lactide)) to form a composite material, respectively. The pc-w-HA/PLLA composite showed better mechanical properties (tensile strength of the pc-w-HA/PLLA composite was 22.3% higher than that of w-HA/PLLA), which could be attributed to mainly two factors including the structure preservation of w-HA bundles and pseudorotaxane linkage between PLA-cyclodextrin and PLLA. The MSCs adhesion of the pc-w-HA/PLLA composite was much better due to balanced hydrophilicity/hydrophobicity and surface roughness. This surface modification method could provide a new and effective strategy for the preparation of bioresorbable composite material with great bioactivity and mechanical property, which has great potential in the medical device industry.