Introduction of 6-Formylcytidine into a Myb Binding Sequence

Abstract

Single 6-formylcytidine was introduced into a oligonucleotide duplex (23 mers) as a substitute for thymidine in the Myb binding sequence of 3-TTGAC-5′. The modified duplex showed Tm of 67°C, which was six degrees lower than the Tm of the native duplex. Binding affinity of the 23-mers to the Myb protein was estimated by electrophoretic mobility shift assays, and the binding was almost completely abolished.