Introducing Soybean Oil in Semen Extenders of Awassi Rams

Nameer Al-Biaty,Abbas F Kareem,Safaa Abed,Safaa Almaliki,Hazim J Alobaidi,Mohammed Mukhlif

doi:10.24126/jobrc.2018.12.1.552

Abstract

The aims of the study was to investigate the possibility of using an plant lecithin; commercial soybean oil (SO) directly in the components of semen extenders of Awassi rams, storage of semen in chilled technique and the effects of dilution, cooling and storage periods on semen quality. Semen was collected weekly from four Awassi rams by electro- ejaculator. Pooled samples were divided to six equal aliquots and diluted by Citrate egg yolk extender at 37˚C. Treatments were designed on the base of control extender containing 25% egg yolk and four treatments containing different addition of SO (12.5, 25, 37.5, and 50%) and combination treatment of 12.5% egg yolk + 12.5% SO. Treatment tubes were cooled to 5˚C and stored for three days. Semen was evaluated as raw, diluted, cooled and after storage in refrigerator (5˚C) for 1, 2 and 3 consecutive days. Results showed that there were no significant differences among all treatments in progressive motility, dead sperm %, abnormal sperm % and acrosome defect %, while pH were found to be significantly declined (p≤ 0.05) in control group. Significant effects of dilution, cooling, and storage period have been demonstrated with steadily significant deterioration (p≤ 0.05) for all studied characteristics when motility and pH declined while sperm abnormality, dead, and acrosome defect percentages increased. The results clearly indicated successful of using different levels of SO (plant source) as lecithin source instead of egg yolk (animal source of lecithin) without any impacts on the biological characteristics of Awassi ram semen and the process of dilution, cooling and storage periods have deterioration effects on semen quality.

Highlights

‫ يخففبث‬،ٍ‫ كببش انعىاس‬،ٌ‫ يذة انخض‬،‫ صَج فىل انصىَب‬،ٍُ‫ انهُسُث‬:‫انكهًبث انذانت‬ Introduction The successful fert ilization and storage of ram semen required sperm of high motility, free of abnormality, intact protoplasmic membrane and chromat in [1,2]
When semen was diluted and cooled to prepare for storage, some phospholipids lost from cytoplasmic membrane [3] due to re -regulation process take place under the impact of cooling [4] wh ich means that fertilizat ion will be declined, the lost particles have to co mpensate by adding good source of phospholipids
Egg yolk contains salt and granules adhered at spermatozoa me mb rane [15], microbia l conta mination is one of the dangerous factor affected semen storage [16,17], in addition to the stimulation of antibody production in circular and genital systems of female due to antigen role o f egg yolk [18], there are an interaction of egg yolk triglycerides with lipase enzy me found in the seminal plas ma of bucks wh ich produce p oisons for the spermatozoa [19,20]

Summary

Introduction

‫ يخففبث‬،ٍ‫ كببش انعىاس‬،ٌ‫ يذة انخض‬،‫ صَج فىل انصىَب‬،ٍُ‫ انهُسُث‬:‫انكهًبث انذانت‬ Introduction The successful fert ilization and storage of ram semen required sperm of high motility, free of abnormality, intact protoplasmic membrane and chromat in [1,2]. Many advantages have been demonstrated in egg yolk including phospholipids, antioxidants, cholesterol and poly unsaturated fatty acids [12,13,14]. In spite of these advantages, there are many disadvantages recorded in the literatures. The aims of this study are to investigate the possibility o f using SO (p lant source of lecithin) d irectly in the preparation of extenders instead of egg yolk lecithin EYL(animal source), storage of semen in chilled technique (5 ̊C) and the effects of dilution, cooling and storage periods on semen quality of Awassi rams

Objectives

Methods

Results