Abstract
The gut microbiota modulates overall metabolism, the immune system and brain development of the host. The majority of mammalian gut microbiota consists of bacteria. Among various model animals, the mouse has been most widely used in pre-clinical biological experiments. The significant compositional differences in taxonomic profiles among different mouse strains due to gastrointestinal locations, genotypes and vendors have been well documented. However, details of such variations are yet to be elucidated. This study compiled and analyzed 16S rRNA gene-based taxonomic profiles of 554 healthy mouse samples from 14 different projects to construct a comprehensive database of the microbiome of a healthy mouse gastrointestinal tract. The database, named Murine Microbiome Database, should provide researchers with useful taxonomic information and better biological insight about how each taxon, such as genus and species, is associated with locations in the gastrointestinal tract, genotypes and vendors. The database is freely accessible over the Internet.
Highlights
Mouse, Mus musculus, has long been used for biological studies as a mammalian model organism due to its low cost, short generation time and small size [1,2]
We present the murine microbiome database (MMDB), which contains 16S rRNA gene-based microbiome taxonomic profiles (MTPs) of the healthy mouse gut microbiota with manually curated metadata
To assess overall relationships among the samples held in the database, the beta-diversity was calculated and compared using statistical values. These results provide further insights into the relationship between the healthy mouse gut microbiome and the various conditions in terms of taxonomic compositions and species diversities within a specific condition or between the different conditions
Summary
Mus musculus, has long been used for biological studies as a mammalian model organism due to its low cost, short generation time and small size [1,2]. Mice have been extensively used in microbiome-related studies which demonstrated a clear correlation between the mouse gut microbiome and host immune response [8,9,10,11,12,13,14,15,16,17,18]. Inbred laboratory mouse strains, including C57BL/6, have been widely used in biological experiments and pre-clinical testing, it is difficult for most researchers to comprehend the usual taxonomic make-up of mouse gut microbiota and the frequencies of each species or genus in different conditions, such as locations in the gastrointestinal (GI) tract, genotypes or vendors/environments [20,21,22,23,24]. There are no curated databases for the mouse gut microbiome
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
