Abstract

We describe a computer tutorial that introduces the concept of the steady state in enzyme kinetics. The tutorial allows students to produce graphs of the concentrations of free enzyme, enzyme–substrate complex, and product versus time in order to learn about the approach to steady state. By using a range of substrate concentrations and rate constants, students are able to simulate enzyme kinetics and produce Lineweaver–Burk plots. The tutorial is intended for use in third- or fourth-year undergraduate biochemistry courses.

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