Abstract
Spectrophotometer is modern analytical instrument for analysis the amount of substance based on the color change. However, for high school laboratory practice, only few schools had this instrument for chemical analysis. There are many studies reported the use of daily life electronic for colorimetric analysis such as smartphone, scanner and digital camera. This work demonstrated the used of document scanner for colorimetric glucose determination for easy learn the daily life instrument as analytical device. The color detection was based on the reaction of glucose with glucose oxidase enzyme to produce H2O2. The H2O2 was then reacted with TiOSO4 to produce yellow color. The color formation was captured with a document scanner and the digital image was analyzed using ImageJ software. The result of the used of document scanner showed Horwitz Ratio of 0.40, recovery of 98.4%, linear responses from 0.5 mM to 3.0 mM (r = 0,9989), limit of detection of 0.15 mM and limit of quantification of 0.40 mM.
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