Abstract
A hydroponic growth procedure which enables the intrinsic 42 Ca-labelling of green beans (Phaseolus vulgaris L) has been developed. Whole-plant Ca influx was measured from planting through the period of pod expansion for a crop of 60 plants grown hydroponically in a 3 m 2 area. Total Ca influx was 10.1 mmol per plant ; 35% of this influx occurred during the period of pod growth. Plants were labelled with 42 Ca via the nutrient solution during the period of pod expansion ; isotopic composition of harvested pods was nearly identical to that of the administered solution. Recovery of isotopic tracer at harvest was as follows : pods 8.4% ; leaves, 61.1% ; stems, 17.1%, roots, 9.0%. Pod yield was 98.8 g FW per plant. 42 Ca-labelled green beans can be used safely to investigate Ca bioavailability in humans.
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