Intrinsic Triple-Enzyme Mimetic Activity of V6O13 Nanotextiles: Mechanism Investigation and Colorimetric and Fluorescent Detections

Abstract

Nanozymes that display multiple activities have received increasing attention because they can be applied more widely than single-active nanozymes. In this paper, we demonstrated that the as-prepared V6O13 nanotextiles (NTs) with interlaced network structure possess triple-enzyme mimetic activity for the first time. The intrinsic oxidase-like, peroxidase-like, and catalase-like catalytic behavior of V6O13 NTs was investigated. Michaelis–Menten kinetics and high affinity for TMB and H2O2 was observed, indicating that V6O13 NTs can be used as enzyme mimics. These multiactivity V6O13 NTs were robust, inexpensive, and easily prepared. Based on the oxidase-like and peroxidase-like activity, a colorimetric biosensor for the glutathione (GSH) detection and a fluorescent system for the detection of H2O2 and glucose were established. The detection of GSH and glucose in health supplements and human serum samples was successfully applied through the proposed method.