Abstract

Southern bean mosaic virus in spherical plant viruses possesses an intrinsic (structural) anisotropy. According to the light scattering theory of Rayleigh and Gans for optically anisotropic spheres, both the refractive index increment and the scattering ratio at a 90° scattering angle of small spheres depend on the intrinsic anisotropy and the relative refractive index of the sphere to the solvent. By using these theoretical expressions the intrinsic anisotropy parameter of southern bean mosaic virus particle in compact spherical state was calculated with the appropriate experimental values. The principal particle refractive indices along the major and minor axes obtained were 1.73 and 1.56 respectively, and the mean refractive index was 1.60.

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