Intrinsic modifying-antibiotic activity of a liposomal structure against MRSA and other MDR bacteria

Abstract

Abstract This work aimed to develop and characterize liposomes and evaluate their antimicrobial carrier activity. In the preparation of liposomes, dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylserine (DPPS) and cholesterol (COL) in a proportion of 5: 3: 1 were used. Vesicle diameter, Polydispersion Index and Zeta Potential analyzes were performed. Atomic Force Microscopy (MFA) and Scanning Electron Microscopy (SEM) were used to determine the shape and morphology of the liposome surface. Microbiological assays were performed against standard and multiresistant bacteria Staphylococcus aureus and Escherichia coli. For the liposome modulating effect test without incorporation with norfloxacin and gentamicin reference antibiotics, the fraction was used at a subinhibitory concentration (MIC/8) and the drugs were microdiluted. The work follows a prospective approach to develop a possible liposomal source with antimicrobial reader properties.