Intrinsic and commissural connections of the rat inferior colliculus.

Abstract

This study analyzes the distribution of the intrinsic and commissural fiber plexuses originating in the central nucleus of the inferior colliculus in the rat. The anterograde tracer Phaseolus vulgaris-leucoagglutinin (PHA-L) was injected iontophoretically at different places along the tonotopic axis of the central nucleus and visualized immunohistochemically. In coronal sections the terminal fields of axons originating at each injection site are seen to create four well-defined bands across the rostrocaudal extent of the inferior colliculus, two in the ipsilateral and two in the contralateral side. The "ipsilateral main band" extends dorsomedially and ventrolaterally from the injection site, in register with the known isofrequency contours of the central nucleus, spanning this nucleus and extending into the dorsal cortex of the inferior colliculus. The "ipsilateral external band" is located in the external cortex, where it is oriented dorsoventrally, slightly oblique to the pial surface. In caudal sections, the ventral portion of these two bands appear to join. The two bands in the contralateral inferior colliculus occupy a symmetric position to those of the ipsilateral side, forming a mirror-like image. The position of the four bands changes as the position of the injection site is varied along the frequency gradient axis of the central nucleus. After ventromedial (high frequency area) injections, the main band is ventral and medial, and the external band ventral and lateral. After more dorsolateral (lower frequency) injections, the main band is more dorsal and lateral, whereas the external band is more dorsal but more medial. Thus, the change in the position of the external band is separate and opposite to that of the main band. We suggest that the main bands represent isofrequency contours. Since the projection from the central nucleus to the external cortex of the inferior colliculus also appears to be tonotopic, we also propose a tonotopic organization for the external cortex. The main bands overlap the terminal field of the lemniscal fibers in the central nucleus; thus, it is concluded that the intracollicular fibers contribute to the formation of the known fibrodendritic laminae of the central nucleus. A possible role in preservation of frequency information and integration of other different acoustic parameters is proposed for the main bands. The external bands could participate in polysensory integration, and the commissural connections could be involved in hitherto unknown stages of binaural processing of sound. Based on our results, several modifications are proposed for delineating the subdivisions of the inferior colliculus.