Abstract
During the last few years our group has focus on assessing mammalian embryos viability in order to improve methods currently used in different assisted reproductive techniques: embryo transfer procedures, in vitro embryo production, cultivation, and cryopreservation techniques. Besides morphometric characterization of in vivo and in vitro produced embryos, after embryo recovery/fertilization and during the in vitro cultivation we also applied different intravital staining tests. Fluoresceine diacetate (FDA) is a non fluorescent analog of fluoresceine, which can easily penetrate the cell membrane of the living cells and under cellular esterase activity is hydrolyzed to fluoresceine, which emit green fluorescence while exposed to a fluorescence source of 494-518 nm wave length.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Animal Science and Biotechnologies
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
