Intravenous Injection of Serratia marcescens Nuclease in Mice Induced Elevation of Synthetized and Secreted into the Serum Extracellular Ribonucleases

Abstract

Deoxyribonucleases and ribonucleases are interest as potential anticancer compounds. Along with mammalian DNases and RNases the cancer suppressive effect was demonstrated for expressing the both activities nuclease from bacterium Serratia marcescens. The aim of the study was to examine how the appearing small amount of S.marcescens nuclease in blood can influence the level of serum DNase and RNase activities. Intravenous injection of S. marcescens nuclease in mice at the dose of 0.5µg /g of mouse was found to induce intensive rising RNA degrading activities in the serum. The induced activities differed from S.marcescens nuclease by the products of RNA degradation and requirement of magnesium cations. S.marcescens nuclease at the dose of 0.5µg /g of mouse did not both induce acute adverse effects and influenced on physical appearance and behavior in mice that evidenced a lack of the nuclease toxicity in vivo.