Intravenous administration mode for lead inclusion development in mouse tissues

Abstract

B6CF1 mice were injected with a 80 mg lead (Pb)/kg. One week later, the liver retained about 0.13 mg Pb/g or 9.8 ± 1.5% of the injected dose (ID)/g fresh weight (mean ± SE, n = 4). This level decreased to 3.9 ± 0.6% ID/g ( n = 5) after 2 weeks. Intranuclear Pb inclusions averaging 1.6 ± 0.1 μm in diameter ( n = 10) were present within parenehymal hepatocytes. Intravenous (i.v.) injections of 2.6 μCi were adequate for autoradiographic localization of Pb deposits within the liver. One week after Pb injection, most tracts were present over endothelial lining cells of the hepatic sinusoids; however, Pb, deposited within intranuclear inclusions of parenehymal hepatocytes was inadequate for tract develop- ment. Therefore, i.v. administration of 80 mg/kg Pb resulted in the formation of inclusions within nuclei of parenchymal hepatocytes of B6CF1 mice.