Abstract
Resting B (rB) cells and splenic dendritic cells (DCs) were purified from splenocytes as described. A typical preparation of rB cells as analysed by flow cytometry was found to be 95.7% major histocompatibility class (MHC) II, 1.2% CD80, 2.5% CD86, and 92.3% sIg. A typical preparation of splenic DCs was 92.2% MHC II, 68.8% CD80, 88.2% CD86, 68% N418, and 9.4% sIg. In functional assays, DCs but not rB cells were able to stimulate proliferation of allogeneic T cell and generate cytotoxic T cells. T cells were made from spleen using a nylon wool column as described and were sorted negatively by FACS Vantage with turbo sort option (Becton Dickinson, Mountain View, Calif). Typical preparations as analysed by FACS was found to be .90% CD3, ,0.5% IA, and ,1% Ig.
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