Abstract
Ozone is widely used to relieve chronic pain clinically, but the precise mechanisms governing its action have yet to be elucidated. The present study aimed to investigate the mechanisms underlying the pain‑alleviating effect of ozone in the chronic constriction injury (CCI) model of sciatic nerve in rats. Pain behaviours of rats were assessed by mechanical allodynia and thermal hyperalgesia. The expression of spinal glutamate receptor6(GluR6) and NF‑κB/p65 was detected by western blotting and reverse transcription‑quantitative PCR. Meanwhile, the expression of spinal IL‑1β, IL‑6 and TNF‑α was detected by ELISA. GluR6 short interfering (si)RNAs were used intrathecally immediately following CCI once per day. Ozone (10,20or30µg/ml) or oxygen was injected intrathecally on day7 after CCI. The expression level of spinal GluR6 increased on day3 and reached a peak on day7 after CCI. The expression level of spinal IL‑1β, IL‑6, TNF‑α and NF‑κB/p65 also increased on day7 after CCI. In addition, pre‑intrathecal injection of GluR6 siRNAs inhibited pain behaviours and suppressed the expression of spinal GluR6, IL‑1β, IL‑6, TNF‑α and NF‑κB/p65 in CCI rats on day7. Intrathecal injection of ozone was also observed to inhibit pain behaviours and suppress the expression of spinal GluR6, IL‑1β, IL‑6, TNF‑α and NF‑κB/p65 in CCI rats on day7. The present study suggested that GluR6 served a pivotal role in neuropathic pain and that intrathecal injection of ozone may alleviate neuropathic pain via the GluR6‑NF‑κB/p65 signalling pathway.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.