Abstract
Objective: To assess the role of intrathecal antibody production (ITAbP) in a murine model of multiple sclerosis(MS).Background: ITAbP is a hallmark of MS, but is poorly understood. Infection of mice with Theiler's murine encephalomyelitis virus (TMEV) results in a progressive disabling demyelinating disease resembling MS, but the role of antibody in central nervous system(CNS) injury in this model is unknown.Design/Methods: ITAbP in TMEV‐induced demyelinating disease(TMEV‐IDD) was measured using ELISpot for IgG‐producing cells and RT‐PCR for IgG1 mRNA, viral load was determined by RT‐PCR for TMEV RNA, and CNS injury was measured by Rotarod analysis of infected mice. Demyelination and immunoglobulin deposition were assayed histologically in the spinal cord by luxol fast blue and immunofluorescent staining, respectively.Results‐Robust ITAbP was present in 85% of infected mice and correlated positively with CNS injury and viral load. Demyelination and immunoglobulin deposition in injured tissue was prominent in mice with ITAbP.Conclusions/Relevance: ITAbP and immunoglobin deposition is highly associated with CNS injury in a murine viral model of MS. These data provide evidence that intrathecally produced antibody may contribute to CNS injury.
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