Intraspecific variation in testosterone-induced neuroplasticity in two canary strains

Abstract

Temperate zone songbird species, such as the canary (Serinus canaria), can serve as model systems to investigate adult seasonal plasticity in brain and behavior. An increase in day length, experienced by canaries in the early spring stimulates gonadal recrudescence and an associated increase in circulating testosterone concentrations. This increase in plasma testosterone results in marked morphological changes in well-defined neural circuitry regulating reproductive behaviors including birdsong as well as behavioral changes such as increases in song length and complexity. An obvious measure of plasticity in neural morphology can be assessed via changes in brain nuclei volume and testosterone actions on a number of cellular features including the integration and incorporation of new neurons in the adult canary brain. Previous work in our lab suggests that there may be systematic intraspecific variability within canaries in testosterone-induced adult neuroplasticity. For example, the song nucleus HVC increases in size in response to testosterone in male canaries but we found that males of the American Singer strain exhibited minimal and variable responses as compared to other canary strains such as the Border canary strain, which is thought to be closer to wild type canaries. In this study, we systematically compared the effects of testosterone on the volume of song nuclei and the number of new neurons as assessed with the neurogenesis marker doublecortin in American Singer and Border canaries. We found more pronounced testosterone-induced neuroplasticity in the Border strain than the American Singer. These data suggest that the process of selection for certain strain phenotypes is also associated with significant changes in hormone-regulated brain plasticity.