Abstract
Citri Reticulatae Pericarpium, the desiccative mature peel of Citrus reticulata Blanco or its cultivated varieties, is a national geographical indicated product that has the concomitant function of both medicine and foodstuff. The primary source of Citri Reticulatae Pericarpium is Citrus reticulata “Chachi,” called “Guang chenpi,” while it differs in variety, propagation, grafting rootstock, and tree age, and the hereditary stability of its biological information between intraspecific plants is worthy of our attention. Homologous analysis result of 4 DNA barcodings in the ribosome or the chloroplast showed that the homology of them (ITS2, rbcl, matK, and psbA-trnH) of 22 samples was 100.00%, 99.97%, 99.99%, and 99.81%, respectively, which indicated that 4 DNA barcodes maintained a high degree of genetic stability in Citrus reticulata “Chachi.” Also, ITS2 was considered to identify Citrus reticulata “Chachi” from other varieties because it presented not only low variability within a certain taxon but also a high level of interspecies variability. Simultaneously, variant site detection of Citrus reticulata “Chachi” was analyzed by comparing with the reference Citrus reticulata genome, and 2652697 SNP sites and 533906 InDel sites were detected from whole-genome resequencing data of 22 samples, providing the data resources and theoretical foundation for the future study about the relevant molecular makers of “Guang chenpi.”
Highlights
Citri Reticulatae Pericarpium (CRP), a traditional Chinese medicine that has the concomitant function of both medicine and foodstuff, is the desiccated mature peel of Citrus reticulata Blanco or its cultivated varieties. ereinto, Citrus reticulata “Chachi,” the main cultivated variety of Citrus reticulata Blanco, is the primary source of genuine Chinese medicinal materials “Guang Chenpi” [1]
Our work indicated that 4 DNA barcodes (ITS2, rbcl, matK, and psbA-trnH) maintained a high degree of genetic stability in Citrus reticulata “Chachi” of different propagation methods, different tree ages, different varieties, and different rootstocks
To excavate more useful molecular markers for distinguishing Citrus reticulata “Chachi” between intraspecific plants or other cultivars, DNA barcoding analysis and variant type detection of the Citrus reticulata “Chachi” were studied for the first time in this investigation, which laid a special foundation for the biological information analysis of Citrus reticulata “Chachi” for the national geographical indicated product Citri Reticulatae Pericarpium
Summary
Citri Reticulatae Pericarpium (CRP), a traditional Chinese medicine that has the concomitant function of both medicine and foodstuff, is the desiccated mature peel of Citrus reticulata Blanco or its cultivated varieties. ereinto, Citrus reticulata “Chachi,” the main cultivated variety of Citrus reticulata Blanco, is the primary source of genuine Chinese medicinal materials “Guang Chenpi” [1]. Citri Reticulatae Pericarpium (CRP), a traditional Chinese medicine that has the concomitant function of both medicine and foodstuff, is the desiccated mature peel of Citrus reticulata Blanco or its cultivated varieties. Ereinto, Citrus reticulata “Chachi,” the main cultivated variety of Citrus reticulata Blanco, is the primary source of genuine Chinese medicinal materials “Guang Chenpi” [1]. A large number of studies have focused on the chemical compounds in CRP by morphological identification, microscopic identification, TLC, UV, HPLC, GC-MS, and LC-MS [8,9,10], while these method do not distinguish well among different cultivars or among different varieties of Citrus reticulata “Chachi.” As an emerging method of identification of food and natural medicinal materials, molecular marker (DNA barcoding, SNP, and InDel) has considerable untapped potential in the quality control and origin identification of food and medicinal materials.
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