Intraspecific competition favors ant–plant protective mutualism

Abstract

AbstractThe dependency of the anti‐herbivore defense on ant–plant protective mutualism often varies depending on abiotic and biotic conditions. Although intraspecific competition is a primary interaction between neighboring plants, its effects on ant–plant mutualisms have yet to be sufficiently elucidated. In order to determine the effects of intraspecific competition and competitor genotype on ant–plant mutualisms, I conducted competition and ant‐removal experiments and examined their effects on damage to the leaves of Urena lobata var. tomentosa plants. I found that larger numbers of worker ants visited the plants growing with non‐siblings than plants growing alone and that plants growing with non‐siblings had a higher shoot to root ratio and secreted greater volumes of extrafloral nectar than plants growing alone and/or with siblings. Under the presence of both sibling and non‐sibling competitors, I observed that when ants were removed from plants, those grown with conspecific neighbors were characterized by a higher percentage of damaged leaf area than plants harboring ants. The effect of ant exclusion on leaf damage was more pronounced in plants grown with non‐siblings than those grown near siblings. However, when the plants were grown alone, I detected no significant difference in percentage leaf damage between the ant‐excluded and ant‐harboring plants. The results indicate that neighboring plants can exert strong effects on ant–plant protective mutualisms, thereby highlighting the need to take into consideration plant–plant interactions in studies on these mutualistic associations.