Abstract
Poliovirus-infected FL cells, incubated at 28°C, were examined by electron microscopy. The immunofluorescent antibody technique was employed for detection of the intracellular viral antigen. Electron micrographs of cells from infected cultures showed both extensive cytoplasmic and nuclear alterations. Virus crystals were observed not only in the cytoplasm but also in the nucleus of cells 14–20 hours after infection. Intracytoplasmic crystals consisted of dense particles, approximately 27 mμ in diameter, suggesting mature virus particles, while intranuclear crystals were composed of empty particles with a less dense central area. Nuclei were stained specifically by fluorescent antibodies as small brilliant foci, suggesting that the intranuclear crystals possess the same antigenicity as the viral antigen. As judged by their size, structure and antigenicity, the intranuclear virus-like particles probably represent an incomplete form of the virus.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
