Intranasal inoculation with an adenovirus vaccine encoding ten repeats of Aβ3–10 induces Th2 immune response against amyloid-β in wild-type mouse

Abstract

To develop a safe and efficient vaccine for the treatment of Alzheimer's disease, we constructed a novel adenovirus vaccine encoding ten repeats of Aβ3–10 and CpG motif as an adjuvant and investigated the immune response in BALB/c mouse after intranasal inoculation with this vaccine. The Ad-10×Aβ3–10-CpG induces an IgG1 predominant humoral response and production of IL-4 and IL-10 in splenocytes in vitro, indicating a Th2-polarized immune response. Stimulation of splenocytes with Aβ3–10 peptide induces robust proliferation but not with full-length Aβ42 peptide, demonstrating that Ad-10×Aβ3–10-CpG does not induces Aβ42 specific T cell immune response. The findings raise the possibility that the adenovirus vaccine Ad-10×Aβ3–10-CpG could be a safe and effective alternative for immunotherapy in Alzheimer's disease.