Intranasal immunization with Cry1Ac activates differentially lymphocytes in NALT as in NP. (41.16)

Abstract

Abstract Cry1Ac from B. thuringiensis is a potent mucosal immunogen and adjuvant. Via i.n. route Cry1Ac induces significant antibody responses and is able to improve vaccination against nasal invading pathogens, but their effects on nasal lymphocytes have not been determined. NALT and NP are considered as inductive and effector sites, respectively. We investigated the effect of i.n. immunization with Cry1Ac in the expression of CD69 and CD25 in lymphocytes of NALT and NP. Besides we analyzed effect in the proportion of T cells producing IL2, IL4, IL5, IL10, IFNγ and TNFα. Immunization with Cry1Ac increase considerably the expression of activation markers in both nasal tissues. But as expected for a mucosal effector site, NP contained major proportions of B220, T CD4 and T CD8 cells expressing CD25 and CD69 in comparison to NALT, Likewise, Cry1Ac increased the proportion of T cells spontaneously producing IL2, IFNγ, and IL4, and the effect was higher in NP than in NALT. Present study provides evidence that intranasal immunization with Cry1Ac activates differentially lymphocytes in NALT as in NP. These data support that Cry1Ac is a potent immunogen and a valuable study tool to the induction of nasal immune responses. The new information presented, is important, as it is necessary to have a thorough understanding of the unique molecular and cellular properties of the nasal immune system for the development of successful nasal vaccines CONACyT 43102 and UNAM DGAPA PAPIIT IN213903 grants