Abstract
After sonication, yeast mitochondrial DNA analyzed on CsCl density gradient shows several components, of which some have a higher and others a lower buoyant density than that of the undergraded mitochondrial DNA (1.684 g/cm 3). Occurrence of different components was also observed after sonication of mitochondrial DNA prepared from a cytoplasmic respiratory-deficient mutant (DM 1). It was shown (1) that DM 1 mitochondrial DNA has a buoyant density of 1.671 g/cm 3 corresponding to A-T and G-C contents, respectively of 96% and 4%. The intramolecular heterogeneity observed after sonication suggests that the G-C content could not be uniformly distributed along the DNA molecule, but could be localized in a limited region of the molecule. If this is true, this DNA could still have genetic information.
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