Abstract
Objective To investigate the mechanism of soluble epoxide hydrolase (sEH) in the pathological process of liver fibrosis in rats with cirrhosis portal hypertension. Methods Portal pressure was measured in rats. The expression levels of sEH protein, α-smooth muscle actin (α-SMA), type Ⅰ collagen (COLⅠ)and cytochrome protein in liver tissues of rats in 4 groups were determined by Western blotting and/or immunohistochemistry. The expression of Epoxyeicosatrienoic acid (EET) and was detected by liquid chromatography tandem mass spectrometry (LC-MS/MS) in 4 groups. Results (1) The portal pressures of each group were (6.65±0.82), (5.94±0.91), (16.54±1.42) and (12.62±1.31) mmHg (1 mmHg=0.133 kPa). The protein expression levels of sEH, α-SMA, COLⅠ of liver of the experimental control group were significantly increased, while they were decreased in the trans-4-[4-(3-adamantan-1-yl-ureido)-cyclohexyloxy]-benzoic acid (t-AUCB) group. The expression levels of EET in portal hypertensive rats was decreased significantly and revived after t-AUCB treated. Conclusion t-AUCB can reduce the sEH levels in the livers, which is able to improve expression of EET. Key words: Liver cirrhosis; Portal hypertension; Soluble epoxide hydrolase; Epoxyeicosatrienoic acids
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