Intraglandular transport of 125I-glandular kallikrein in the rat submandibular salivary gland.

Abstract

The transport of radiolabelled rat submandibular gland kallikrein was studied after local administration to the resting and activated rat submandibular gland. The iodinated kallikrein was electrophoretically, immunologically, and biologically indistinguishable from the intact enzyme. After intraductal and intraglandular application the radioactivity in venous effluent was quantitated and characterized. As judged by gel-filtration 125I-kallikrein in venous effluent eluted at a position similar to that seen when the iodinated enzyme was mixed with plasma, but earlier than the elution of 125I-kallikrein in buffer. In plasma, therefore, glandular kallikrein is probably bound to macromolecules. The radioactive fractions in venous effluent did not contain free iodine. Maximum concentration of 125I-kallikrein in venous effluent of resting glands was repeatedly reached about 20 min after intraductal administration. Moreover, the ductal epithelium represented the main permeation barrier since after intraglandular application the maximum venous 125I-kallikrein concentration was reached almost immediately. In activated gland (parasympathetic and sympathetic nerve stimulation), the venous 125I-kallikrein concentration was inversely related to glandular blood flow. We conclude that kallikrein present in the duct lumen or in the interstitium is able to reach the circulation, thereby making possible the local generation of plasma-kinins.