Intracranial self-stimulation as a positive reinforcer to study impulsivity in a probability discounting paradigm

Abstract

Probability discounting is used to study risky decision-making in humans and rodents. In these paradigms, the subject chooses between a small reward that is always delivered and a large reward that is delivered with varying probabilities. Risk-taking is defined as a preference for the large, uncertain reward. The aversive consequence associated with this task involves choosing the large reward and not obtaining it. To study this form of impulsivity in rodents, food reinforcement is commonly used. Using this reinforcer, and the need to food-deprive rodents to enhance task performance, may be problematic in rodent models that exhibit eating disorders, in pharmacological assessments that alter feeding, and for assessments of the neurocircuitry that is engaged by both feeding and risk-taking. We reveal here that electrical intracranial self-stimulation (ICSS) can be used as the positive reinforcer in risk assessments (i.e., probability discounting). ICSS was selected as it is rapidly acquired, the operant procedures are retained for months, and no tolerance or satiety develops to the reinforcer; thus, ICSS can be used in multiple test sessions in a repeated measures design. We developed an efficient, standardized, six phase ICSS-mediated protocol that allowed for the assessments of risk-taking in a probability discounting task. We demonstrated that the discounting behavior remained stable for several weeks. The value of this protocol is discussed in terms of practical as well as theoretical advantages of using ICSS-mediated reinforcement.