Abstract
Methods circRNA expression was analysed in six cerebrospinal fluid (CSF) samples from three patients of the infectious and noninfectious phases using an Arraystar Human circRNA Array. Differentially altered circRNAs were validated by quantitative real-time polymerase chain reaction (qRT-PCR) in the 66 CSF samples of 33 patients of the infectious and noninfectious phases. t-test was used for statistical analysis. A bioinformatics analysis was employed to investigate the function mechanism of the circRNAs. Results Firstly, 142 circRNAs were found significantly different in 6 CSF samples of the infection and noninfection phases of 3 patients. Fourteen circRNAs with the top largest fold changes were chosen from the 142 circRNAs for PCR validation in the same 6 CSF samples of 3 patients. Three circRNAs were selected to be validated in 60 CSF samples of 30 patients using the PCR test. In infection CSF, an upregulated hsa_circRNA_402632 and downregulated hsa_circRNA_008636 and hsa_circRNA_405481 were confirmed by PCR test. A bioinformatics analysis was used to investigate the function mechanism of the 3 circRNAs. hsa_circRNA_402632 is enriched in the insulin resistance pathway, the FoxO and AMPK signaling pathways are the most important pathways for hsa_circRNA_008636 gene expression, and hsa_circRNA_405481 is enriched in the endometrial cancer signaling pathway, Fc epsilon RI signaling pathway, and TGF-beta signaling pathway. Conclusions hsa_circRNA_402632, hsa_circRNA_008636, and hsa_circRNA_405481 may be potential diagnostic markers for central nervous system infection after neurological surgery.
Highlights
Postoperative central nervous system infection (PCNSI) is a common and serious complication [1,2,3,4,5]
Neither a bacterial culture nor cytological detection of cerebrospinal fluid (CSF) can meet the needs of an early diagnosis of PCNSI. circRNA is an endogenous noncoding RNA with a covalently closed cyclic structure which enables circRNA to resist exonucleases. circRNAs often have high stability and have been widely studied as diagnostic markers in many diseases, including neurodegenerative disorders, diabetes, and various types of cancers [10]
Fan et al investigated the profiles of circRNAs in tilapia meningoencephalitis and identified circRNAs as new biomarkers for meningoencephalitis and neurodegenerative disorders [11]
Summary
Postoperative central nervous system infection (PCNSI) is a common and serious complication [1,2,3,4,5]. CircRNAs often have high stability and have been widely studied as diagnostic markers in many diseases, including neurodegenerative disorders, diabetes, and various types of cancers [10]. CircRNAs as a marker for early diagnosis of PCNSI were investigated by collecting 66 CSF samples from 33 patients in infectious and noninfectious phases. Circular RNA (circRNA) often has high stability and has been widely studied as diagnostic markers in many diseases, including neurodegenerative disorders, diabetes, and various types of cancers. CircRNA was evaluated as a diagnostic marker for central nervous system infection in neurological surgery. Altered circRNAs were validated by quantitative real-time polymerase chain reaction (qRT-PCR) in the 66 CSF samples of 33 patients of the infectious and noninfectious phases. Hsa_circRNA_402632, hsa_circRNA_008636, and hsa_circRNA_405481 may be potential diagnostic markers for central nervous system infection after neurological surgery Conclusions. hsa_circRNA_402632, hsa_circRNA_008636, and hsa_circRNA_405481 may be potential diagnostic markers for central nervous system infection after neurological surgery
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