Intracortical connections of two functional subdivisions of the somatosensory forepaw cerebral cortex of the raccoon

Abstract

The aim of this study was to compare the intrinsic intracortical connectivities of 2 functionally distinct subdivisions of the somatosensory (Sml) forepaw cortex of the raccoon--the somatotopic glabrous skin representation and the more heterogeneous, hairy skin and claw representation of the digits. HRP was injected into one or the other functional subdivision of a particular digit subgyrus of Sml cortex in 10 adult raccoons. The distribution of HRP-labeled neurons and axon terminals in the cortex showed that intrinsic "horizontal" connections exist within and between individual cortical digit zones; the labeling tended to have an oval-shaped configuration that was longer in the mediolateral than in the anteroposterior curvilinear plane. The 2 cortical sectors were found to have different patterns of intracortical projections. The connections of the glabrous skin region of each cortical digit zone were primarily local and confined to that same digit representation. HRP-filled neurons were concentrated near the injection site and decreased in density within the banks and fundi demarcating the injected digit subgyrus; few labeled cells were found in adjoining digit zones. Longer projections to the glabrous subdivision of a particular digit area typically originated from neurons in the heterogeneous subdivision of that same digit area. In contrast, the connections of the heterogeneous region of each digit zone were much more extensive and usually included projections from nonadjacent, as well as neighboring digit zones. The density of HRP-positive neurons declined more gradually with distance from the injection site, and considerable labeling was present in the heterogeneous sectors of adjacent digit zones. The intracortical projections of both functional subdivisions were often, but not always, reciprocal, and the cells of origin tended to be distributed in clusters. The laminar distributions of labeled neurons were similar for both sectors; HRP-filled cells were concentrated more in the supragranular layers, especially in layer III; fewer were found in the infragranular layers, mainly in layer VI and rarely in layer V. These results show that the intrinsic connections of the glabrous cortical subdivisions are fairly localized, whereas those of the heterogeneous cortical subdivisions are more diffuse and highly convergent. The differing intracortical connectional patterns of the 2 sectors are consistent with their contrasting thalamocortical projection patterns and may contribute to the unique functional properties of neurons located within each sector.(ABSTRACT TRUNCATED AT 400 WORDS)