Intracerebral transfection of anti-rabies virus antibodies is an effective therapy for rabies.

Washington C Agostinho,Paulo E Brandão

doi:10.1007/s13365-020-00883-8

Abstract

Rabies is a neurological disease with 100% lethality. Some of the rare human patients who survived after multiple drug treatment had severe sequelae. The present study showed that after 48h of RABV inoculation, mice injected intracerebrally with anti-RABV F (ab')2 plus Bioporter® showed 70% survival compared to the control group, suggesting that transfection of anti-RABV antibodies to the brain may prevent or delay the spread of RABV at an early stage of infection. This result may provide important protocol results in intracellular antibody delivery to prevent the fatal outcome of the disease.

Highlights

Rabies is a zoonotic neurological disease with 100% lethality; some of the few human patients who survived after a multi-drug treatment developed severe motor impairment due to ischaemic encephalopathy followed by necrosis of the hippocampus, cerebellum and cortex [1-2]
The present study showed that after 48 h of RABV inoculation, mice injected by the intracerebral route with anti-RABV F(ab’)2 complexed with Bioporter® Protein Delivery
Reagent (Genlantis) as a transfection agent, showing a morbidity/mortality rate of 30% with a minimum incubation period of seven days, while in the control group a significantly higher (p

Summary

Introduction

Rabies is a zoonotic neurological disease with 100% lethality; some of the few human patients who survived after a multi-drug treatment developed severe motor impairment due to ischaemic encephalopathy followed by necrosis of the hippocampus, cerebellum and cortex [1-2]. Rabies is a zoonotic neurological disease with 100% lethality; some of the few human patients who survived after a multi-drug treatment developed severe motor impairment due to ischaemic encephalopathy followed by necrosis of the hippocampus, cerebellum and cortex [1-. Rabies lyssavirus RABV (Mononegavirales: Rhabdoviridae: Lyssavirus) is a neurotropic virus with a circa 11 Kb negative-sense single-stranded RNA as a genome that codes for the nucleoprotein (N), phosphoprotein (P), matrix protein (M), envelope glycoprotein (G) and the RNA-dependent RNA-polymerase L protein [6], and it is most often transmitted amongst mammals via saliva after an initial local replication in muscle cells that follows to the central nervous system (CNS) via axons [7]. The virus causes enough damage to the brain in a few days that the infection invariably leads to coma and death by cardio-respiratory arrest [8]

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