Abstract
15N-Tempone was synthesized from (NH 4) 2SO 4 and phorone and was used as a spin label probe to study the protoplasmic viscosity of lymphocytes. The intracellular viscosity was determined to be approximately two times the viscosity of water. 14N-Tempone yielded a similar result. The value of the hyperfine coupling constant for 15N-Tempone in lymphocytes was slightly less than that in water. The EPR signal of the intracellular spin label was observed to decrease exponentially as a function of time with a decay constant of 0.25 ± 0.18 min −1.
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