Abstract
Eu-tris(dinaphthoylmethane)-bis-(trioctylphosphine oxide) (Eu-DT) molecules encapsulated by Polystyrene and bis(trimethoxysilyl)decane nanoparticles were prepared via a modified encapsulation-reprecipitation method and show a high sensitivity to sense temperature. After surface modification with poly-L-lysine, the fluorescent nanoparticles obtained a well biocompatibility and low toxicity at a certain concentration. In the physiological temperature range (25-45 °C), the fluorescence of the nanoparticles is rather sensitive to temperature with a sensitivity of -2.6%/°C. The temperature nanosensors and gold nanorods were internalized into living HepG2 cells. The fluorescence intensity of phagocytic nanoparticles decreased with the irradiation of 808-nm laser, which were captured by Epi-fluorescence microscope.
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