Abstract

Features of intracellular action potentials (iAPs) are powerful biomarkers that predict proarrhythmic risks. However, the conventional patch-clamp techniques for measuring iAPs are either laborious and low throughput or not suitable for measuring electrically connected cardiomyocytes. In the last decade, a number of vertical nanoelectrodes have been demonstrated to achieve parallel and minimally invasive iAP recordings. Here, we developed nanocrown electrodes that are mechanically robust and achieve >99% success rates in obtaining intracellular access through electroporation.

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