Abstract
Multiple intracellular proteases were produced by Streptomyces coelicolor throughout growth as surface cultures. Zymography revealed two constitutive, gelatinolytic proteases of approximate molecular masses 32.5 and 36.5 kDa. In addition, transient expression of a large (183.5 kDa) protease preceded aerial mycelium formation and following this, during sporulation, an additional protease of mass 27.5 kDa was produced.
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