Intracellular localization of nigeran in the wall of Aspergillus aculeatus by autoradiography with the electron microscope.

Abstract

Aspergillus aculeatus mycelium was incubated with [(3)H]glucose under conditions that promote nigeran (mycodextran) accumulation (low pH, in the absence of nitrogen). Autoradiography revealed that essentially all the (3)H was localized around the hyphal perimeter. The results strongly support a hyphal wall location for nigeran.