Abstract
AbstractVarious elements including iron play a vital role as micronutrients in growth and development of algae cells. Understanding the process of element uptake, assimilation and utilization of these elements by algae cells are essential for optimizing growth conditions in algae ponds, for cost-effective biomass production. In addition to bulk elemental analysis methods, electron microscopy-based semi-quantitative elemental analysis methods can provide high-resolution spatial information about element distribution in algal cultures for a comprehensive and accurate understanding of the element utilization processes. Toward this goal, we standardized a protocol for localizing various elements within unicellular algae cells using scanning transmission electron microscopy–energy-dispersive X-ray spectroscopy (S/TEM–EDX). We applied this method to study iron uptake and intracellular distribution process by algae cells over a week of cultivation.KeywordsAlgaeIron uptakeMicronutrientsSTEM-EDX
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