Abstract
We have investigated the subcellular localization of the geranylpyrophosphate (GPP) synthase (EC 2.5.1.1) in Lithospermum erythrorhizon cell cultures using a cell fractionation procedure followed by comparison to marker enzyme activities and immunodetection in Western blots. The results demonstrate the localization of the enzyme within the cytosol. This contrasts to the plastidial localization of the GPP synthase in Vitis vinifera. This discrepancy is discussed in regard to the different metabolic pathways in which GPP is involved in these two plant species.
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