Abstract
The movement of intracellular free calcium([Ca2+]i) and phosphatidyl inositol-1, 4, 5-triphosphate (IP3) was studied in bone cells cultured in a low-calcium environment. The [Ca2+]i was 98.0±10.2(n=6)nM/106 cells for the control group (bone cells cultured in control medium) and 21.3±2.8(n=6)nM/106 cells for the low Ca group (bone cells cultured in low Ca medium). After the addition of exogenous CaCl2 to the calibration solution, [Ca2+]i increased significantly more in the low Ca group than in the control group(p<0.01). The IP3 content/2×106 cells was 12.40 pmoles in the control group and less than 0.19 pmoles in the low Ca group. After the stimulation with phospholipase C (PLC), the IP3 content in the bone cells increased markedly more in the low Ca group than in the control group. These findings suggest that a low-calcium environment around cells and organsin vivo may inhibit the intracellular signal tranduction system.
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