Abstract
This study documents a difference between cystic fibrosis human (CF-HTG) and normal human (HTG) tracheal gland cells: the ability of histamine to induce an increase of intracellular free calcium concentration [Ca 2+] i was abnormally reduced in CF-HTG cells. The magnitude of the [Ca 2+] i peak rise in response to histamine is smaller in CF-HTG cells than in HTG cells, and the percentage of CF-HTG cells that increase [Ca 2+] i is decreased compared with HTG cells. In contrast to histamine, the human neutrophil elastase (HNE) stimulation of both CF-HTG and HTG cells generated [Ca 2+] i asynchronous oscillations and the magnitude of the peak [Ca 2+] i response as well as the percentage of responding cells were similar for both groups. By videomicroscopy observations, the secretory response (exocytosis of secretion granules) of CF-HTG cells occurred with HNE, but not with histamine, thus suggesting that [Ca 2+] i asynchronous oscillations may be linked to the exocytosis process in human tracheal gland cells.
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