Intracellular expression of c-Fos protein in various structures of the hypothalamus in electrical pain stimulation and administration of antigens

Abstract

Application of different stimuli activated different hypothalamic structures. Immunohistochemical methods demonstrated changes in the numbers of c-Fos-positive cells in various hypothalamic structures after electrical pain stimulation and i.v. administration of antigens (bovine serum albumin (BSA) and lipopolysaccharide (LPS)). Increases in the numbers of c-Fos-positive cells in all the hypothalamic structures studied occurred after electrical pain stimulation and i.v. administration of antigens (BSA and LPS). The highest level of activation in hypothalamic structures was seen in the anterior hypothalamic nucleus (AHN) and posterior hypothalamic area (PH) after electrical pain stimulation and in the paraventricular nucleus (PVN) and lateral hypothalamic area level 28 (LHA-28) after i.v. administration of LPS. Comparative analysis showed that the level of activation of hypothalamic structures after electrical pain stimulation was significantly greater in the AHN, PVN, LHA, and PH than after i.v. administration of antigens (LPS and BSA). Administration of LPS led to more marked activation of cells in hypothalamic structures APH, PVN, LHA-28, dorsomedial hypothalamic nucleus (DMH), and PH (in terms of the numbers of c-Fos-positive cells) than administration of BSA.