Abstract
The intracellular degradation of extracellular cargo is a process crucial to maintaining cellular health. In the classic model, degradation occurs inside the lysosome. However an alternative pathway has been proposed in which degradation occurs inside the late endosome, with the lysosome serving as an enzyme-storage vesicle. To test this new model, we have disrupted two key lysosomal proteins, LAMP1 and 2, in intact live cells using endoglycosidase H (Endo H). Following Endo H treatment, lysosome mobility was measured using live cell imaging and single particle tracking. Enzymatic activity was measured using fluorogenic substrates. We found that Endo H treatment alters the morphology and mobility of the lysosome and leads to alternate degradation pathways within the cell.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.